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KMID : 1025720120310020044
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Journal of Dental Implant Research
2012 Volume.31 No. 2 p.44 ~ p.48
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Comparative study of proper demineralization time for using the human teeth block as graft material after demineralization by 2% HNO3
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Lee Jong-Seon
Kim Young-Wook
Mo Dong-Yub
Shim Woo-Hyun
Hwang Hong-Jun
Lee Jang-Yeol
Kim Hyoun-Chull
Lee Sang-Chull
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Abstract
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Purpose: Some studies have reported that Autogenous demineralized dentin matrix (ADDM) granules which were demineralized with 2% HNO3 for 20 minutes have osteoinduction and osteoconduction. The aim of this study is finding out the proper demineralization time for using ADDM block as the graft material in comparison previous studying that evaluate the proper demineralization time ADDM granules which were demineralized with 2% HNO3.
Materials and Methods: The enamel of crown was removed and the dentin was shaped as a rectangular parallelepiped for making tooth block (8×8×5 mm). In tooth-blocks, 1 mm diameter holes were formed every 1 mm intervals. Each tooth-blocks were demineralized in 2% HNO3 solution for 1, 2, 3 hours. The change of weight, radiological density and minerals (Ca, P) were measured.
Results: This result has showed that ADDM block which was demineralized by 2% HNO3 is needed to have 2 hours demineralization time. But when applying this to clinical cases, we should consider that it is needed to take subdivided times for the better efficient, economical and stable demineralization time and histological studies through additional animal test.
Conclusion: These results of ADDM blocks were compared with the results of ADDM granules that were already confirmed to evaluate proper demineralization time for using the ADDM block as graft material.
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KEYWORD
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Autogenous demineralized dentin matrix, 2% HNO3, Tooth block
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